Publication on Rauwolfia Vomitoria Extract Against Ovarian Cancer

November 2013 – 'Antitumor Activities of Rauwolfia Vomitoria Extract and Potentiation of Carboplatin Effects Against Ovarian Cancer'

Yu J, Ma Y, Drisko J, Chen Q.
Curr Ther Res Clin Exp. 2013 Dec;75:8-14. doi: 10.1016/j.curtheres.2013.04.001.

Kansas universityDrs. Qi Chen and Jeanne Drisko at Kansas University Medical Center (KUMC) have been interested in ovarian as well as pancreatic cancer and have explored the potential of the Pao Pereira and Rauwolfia vomitoria extracts as alternative therapeutic agents for these cancers.

Like pancreatic cancer, ovarian cancer is frequently diagnosed late; it also develops drug resistance and is similarly difficult to treat. Following the same line of experiments used for testing the Pao Pereira extract against pancreatic cancer the researchers at KUMC studied the anti-ovarian cancer effects of the Rauwolfia vomitoria extract.

They found that Rauwolfia vomitoria was effective against ovarian cancer cells both alone and in combination with carboplatin. The combination decreased tumor size in animal experiments by 87 to 90%.

These dramatic results indicate that in the presence of Rauwolfia, drug resistance of the tumor cells is overcome and the activity of carboplatin is restored. The authors conclude that, 'Rauwolfia vomitoria has potent anti-tumor activity and in combination significantly enhances the effect of carboplatin against ovarian cancer.'

Abstract

rauwolfia and ovarian cancerTumor resistance to platinum-based drugs has been an obstacle to the treatment of ovarian cancer. Extract of the plant Rauwolfia vomitoria has long been used by cancer patients. However, there have not been systematic studies of its anticancer activity. Objective: In an effort to enhance the effectiveness of platinum-based drugs, we investigated the anticancer effect of a Rauwolfia vomitoria extract (Rau), both alone and in combination with carboplatin (Cp). Methods: In vitro cytotoxicity and colony formation were evaluated in several ovarian cancer cell lines. In vivo effects were evaluated in an intraperitoneal ovarian cancer mouse model. The combination of Rau and Cp was assessed using Chou-Talalay's constant ratio design and median effect analysis based on the isobologram principle to determine the combination index values. Results: Rau decreased cell growth in all 3 tested ovarian cancer cell lines dose dependently and completely inhibited formation of colonies in soft agar. Apoptosis was induced in a time- and dosedependent manner and was the predominant form of Rau-induced cell death. Synergy of Rau with Cp was detected, with combination index values o1 and dose reduction index values for Cp ranging from 1.7- to 7-fold. Tumor growth in mice was significantly suppressed by 36% or 66% with Rau treatment alone at a low (20 mg/kg) or a high dose (50 mg/kg), respectively, an effect comparable to that of Cp alone. The volume of ascitic fluid and the number of nonblood cells in ascites were also significantly decreased. Combining Rau with Cp remarkably enhanced the effect of Cp and reduced tumor burden by 87% to 90% and ascites volume by 89% to 97%. Conclusions: Rau has potent antitumor activity and in combination significantly enhances the effect of Cp against ovarian cancer.

©2013. The Authors. Published by Elsevier Inc. All rights reserved.

Download Antitumor Activities of Rauwolfia Vomitoria Extract and Potentiation of Carboplatin Effects Against Ovarian Cancer

Publication on Pao Pereira Extract Against Ovarian Cancer

September 2013 – 'The Plant Extract of Pao Pereira Potentiates Carboplatin Effects Against Ovarian Cancer' published by Informa Healthcare USA, Inc.

Jun Yu, PhD, Qi Chen, PhD.  – ISSN 1388-0209 print/ISSN 1744-5116 –
Pharmaceutical Biology

Kansas universityRecent research conducted at the University of Kansas Medical Center shows that an herbal preparation of Pao pereira, provided by Natural Source International, Ltd. could be extremely helpful.  It concludes that 'In vivo, Pao pereira alone suppressed tumor growth by 79% and decreased volume of ascites by 55%. When Pao pereira was combined with carboplatin, tumor inhibition reached 97% and ascites was completely eradicated. Pao pereira possess potent antitumor activity and could enhance carboplatin effect, and therefore holds therapeutic potential in the treatment of ovarian cancer.'

This is a breakthrough study considering that The American Cancer Society estimates that in 2013, about 22,240 new cases of ovarian cancer will be diagnosed and 14,030 women will die of ovarian cancer in the United States. According to the data, the mortality rates for ovarian cancer have not improved in the forty years since the 'War on Cancer' was declared.

The herbal preparation of Pao pereira long been used by oncologic patients and Integrative Medicine practitioners in South America. This study investigates the anticancer effects of Pao pereira (Pao), either alone or in combination with chemotherapy drugs (carboplatin), in preclinical ovarian cancer models.

The results of the study offer a breakthrough for ovarian cancer research!

Pao pereira selectively inhibited ovarian cancer cell growth but induced apoptosis (programmed cell death) and also Pao pereira greatly enhanced chemo drug cytotoxicity.

In vivo, Pao pereira alone suppressed tumor growth by 79% and decreased volume of ascites by 55%. When Pao pereira was combined with Chimio therapy, tumor inhibition reached 97% and ascites was completely eradicated.
The researchers conclude that Pao Pereira has therapeutic potential in the treatment of ovarian cancer.

Abstract

Herbal preparation of Pao pereira [Geissospermum vellosii Allem (Apocynaceae)] has long been used by oncologic patients and Integrative Medicine practitioners in South America. However, its anticancer activities have not been systematically studied. Objective: To investigate the anticancer effects of b-carboline alkaloids-enriched extract from Pao pereira (Pao), either alone or in combination with carboplatin, in preclinical ovarian cancer models. Materials and methods: Cytotoxicity of Pao (0–800 mg/ml) against different ovarian cancer cell lines and an immortalized epithelial cell line was detected by flow cytometry, MTT assay and colony formation in soft agar. Combination of Pao and carboplatin, a primary chemotherapeutic drug for ovarian cancer, was evaluated using Chou-Talalay's methods. Mice bearing intraperitoneally spread ovarian cancer were treated with 20 or 50 mg/kg/day Pao by i.p. injection. Carboplatin at 15 mg/kg/week i.p. was compared and combined to Pao treatments. Results: Pao selectively inhibited ovarian cancer cell growth with IC50 values of 180–235 mg/ml, compared to 537 mg/ml in normal cells. Pao induced apoptosis dose- and time-dependently and completely inhibited colony formation of tumor cells in soft agar at 400 mg/ml. Pao greatly enhanced carboplatin cytotoxicity, with dose reduction (DRIs) for carboplatin at 1.2–10 fold. In vivo, Pao alone suppressed tumor growth by 79% and decreased volume of ascites by 55%. When Pao was combined with carboplatin, tumor inhibition reached 97% and ascites was completely eradicated. Discussion and conclusion: Pao possess potent antitumor activity and could enhance carboplatin effect, and therefore holds therapeutic potential in the treatment of ovarian cancer.

Download The Plant Extract of Pao Pereira Potentiates Carboplatin Effects Against Ovarian Cancer