67 – RNA-Bound Reverse Transcriptase in Escherichia coli and in Vitro Synthesis of a Complementary DNA

Authors : M. BELJANSKI, M. S. BELJANSKI
Biochemical genetics, 1974, 12, pp. 163-180.

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ABSTRACT: RNA-bound reverse transcriptase can be easily distinguished from RNA-free reverse transcriptase and DNA-dependent DNA polymerase after fractionation of extracts from Escherichia coli on a DEAE-cellulose column. This enzyme is capable of synthesizing a DNA-like product in the absence of exogenously added template provided that all four deoxyribonucleoside 5′-triphosphates are present in the incubation mixture. Removal of RNA from the enzyme by RNase leads to a considerably decreased polymerizing activity. The activity can be restored under appropriate conditions either by RNA originating from the enzyme or by transforming RNA excreted by showdomycin-resistant E. coli. Enzyme-bound RNA has several characteristics already found for the transforming RNA. DNA synthesized by RNA-bound reverse transcriptase is complementary to the enzyme-bound RNA.

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