24 – Protein biosynthesis by a cell-free bacterial system

Proc. Nat. Acad. Sci. Biochemistry, 1958,44, pp. 494-501.. Available in English only ABSTRACT: Particulate preparations of Alcaligenes faecalis, consisting largely of cell membrane fragments, incorporate amino acids into their proteins. This incorporation, which appears to reflect protein biosynthesis, is driven by oxidative phosphorylation and is stimulated by an enzyme, present in the supernatant extract, which has been isolated in highly purified form. The purified enzyme is free of activating enzymes catalyzing the amino acid-dependent exchange of PP32 with ATP and the same seems to be true of the bacterial particles.Read the full publication