Authors : M. BELJANSKI, N. VAPAILLE
Eur. J. of Clin. Biol. Res., 1971, pp. 897-908
Available in French only
Summary in English
ABSTRACT: Asiaticoside, asiatic and madecassic acids affect the binding of L-14V-proline and L-14C-alanine to m-RNA in the presence of any one of the four nucleoside triphosphates and a partially purified bacterial enzyme preparation whose properties have been previously described. The binding of proline to m-RNA, but not its turnover, is inhibited by any one of the triterpenes used, while that of alanine is highly increased only by free asiatic acid. This latter inhibits the turnover of alanine bound to m-RNA thus shifting the reaction towards an accumulation of the “m-RNA-alanine” complex which is an intermediate in the synthesis of oligopeptides in the present system. The binding of L-amino acids to t-RNA in the presence of ATP is not affected by triterpenes. A hypotesis is proposed to account for the effect of triterpenes in mammalian cells in connection with biosynthesis of collagen, a protein rich in proline, hydroxyproline and alanine.