Authors : L. LE GOFF, M. BELJANSKI
IRCS Medical Science, 10, 1982, pp. 689-690.
Available in English only
ABSTRACT: Using a biochemical assay system (Oncotest) we have shown that DNAs from cancerous mammalian and plant cells are destabilized compared to DNA from healthy cells. Cancer DNAs susceptible to the action of carcinogens or other different compounds exhibit in vitro and in vivo a high template activity in comparison to DNAs from healthy cells. We have also shown that alstonine, a plant alkaloïd prepared in our laboratory which selectively binds to DNA from cancer cells prevents DNA in vitro synthesis as well as cancer cells in vivo multiplication in animals and plants. It has slight effect on DNA from healthy cells. We describe here the effects of auxin (IAA) and kinetin (K) (two cell growth and division hormones in higher plant species) and that of alstonine (BG-8) on in vitro synthesis and strand separation of DNAs isolated from cancerous, habituated and healthy plant cells cultures in vitro.