30 – Protein biosynthesis by a cell-free bacterial system. III- Determination of new peptide bonds; requirement for the “amino acid incorporation enzyme” in protein biosynthesis

Authors : M. BELJANSKI
Biochim. Biophys. Acta., 1960, 41, pp. 104-110.

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ABSTRACT: The incorporation of 14C amino acids into protein by Alcaligenes faecalis fragments has been demonstrated. Using 14C valine, it was found that most of the incorporated amino acid was within the peptide chains. Alcaligenes fragments have been partially solubilized by treatment with perfluoro octanoate without affecting the incorporation activity. The soluble fraction obtained after this treatment can be replaced by purified “amino acid incorporation enzymes”.

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